Alternatives to scarce Matrigel and why they are needed.

Matrigel, first developed over 35 years ago has been used in 2D and 3D cell culture, however due to supply limitations, inconsistency of biochemical and mechanical properties and potential for antigenicity, researchers are now looking to other biomaterials for structural support of their cultures.

This ProColl white paper examines the reasons for Matrigel’s limitations and why advances in biotechnology now mean that cell scientists and engineers understandably are demanding more for meeting the needs of 21st century regenerative medicine.

Read the whitepaper here: Mobile users click the link below.

ProColl – Alternatives to Matrigel White Paper

To discuss the purchase, bulk or OEM supply of any of our high performing animal or animal-free collagen products, or for more information, get in contact through the form below.

When using acid soluble collagen, pH neutralisation in buffered conditions causes the collagen molecules to become insoluble and aggregate to form a gel when the pH is neutral. This forms a weak gel and chemical cross-linking is recommended to produce a stiffer gel that is better suited to the cell growth application.

When a higher concentration is used, such as our recommended starting concentration of 6 mg/mL, a robust gel can be formed without the use of a crosslinking agent.

Avoiding the use of crosslinking within your workflow can allow the removal of variables and simplification of the hydrogel environment. This procedure can be carried out at room temperature or incubated to match cell solutions’ optimal conditions.

Read the whitepaper here: Mobile users click the link below.

ProColl – Collagen Gelation Protocol

To discuss the purchase, bulk or OEM supply of any of our high performing animal or animal-free collagen products, or for more information, get in contact through the form below.