When using acid soluble collagen, pH neutralisation in buffered conditions causes the collagen molecules to become insoluble and aggregate to form a gel when the pH is neutral. This forms a weak gel and chemical cross-linking is recommended to produce a stiffer gel that is better suited to the cell growth application.

When a higher concentration is used, such as our recommended starting concentration of 6 mg/mL, a robust gel can be formed without the use of a crosslinking agent.

Avoiding the use of crosslinking within your workflow can allow the removal of variables and simplification of the hydrogel environment. This procedure can be carried out at room temperature or incubated to match cell solutions’ optimal conditions.

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ProColl - Collagen Gelation Protocol

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