ProColl Research Grade Collagen Gelling Protocol – Cross linker free

When using acid soluble collagen, pH neutralisation in buffered conditions causes the collagen molecules to become insoluble and aggregate to form a gel when the pH is neutral. This forms a weak gel and chemical cross-linking is recommended to produce a stiffer gel that is better suited to the cell growth application. When a higher […]

Often the application of collagen requires the production of a structure that is mechanically robust. This can be achieved through a process of cross-linking functional groups on the collagen molecules. When using acid soluble collagen, the collagen molecules become insoluble and aggregates to form a gel when the pH is increased. However, this is a […]

Research by Sheffield University using ProColl’s acid soluble collagen demonstrated its superior performance over gelatin for cell culture. Using ProColl’s collagen resulted in cells with significantly better levels of key bone-growth markers. Calcium content and DNA were significantly higher when cultured on ProColl’s collagen than gelatin. Also, significantly lower collagen production occurred since the closer-to-native […]

Endotoxins unfortunately are often found as contaminants of protein products derived from bioprocesses and because of their strong biological effects at very low concentrations within the human body they are of extreme concern for clinicians and the pharmaceutical industry. In patients even in small quantities endotoxins can result in fever, inflammation, sepsis, tissue damage and […]